标题：The inhibitory effect of polysaccharide from Rhizopus nigricans on colitis-associated colorectal cancer
作者：Song, Ge; Lu, Yan; Yu, Zhidan; Xu, Lei; Liu, Jing; Chen, Kaoshan; Zhang, Pengying
作者机构：[Chen, Kaoshan; Zhang, Pengying] Shandong Univ, Natl Glycoengn Res Ctr, 72 Binhai Rd, Qingdao 266237, Peoples R China.; [Chen, Kaoshan; Zhang, Pengy 更多
通讯作者：Chen, KS;Zhang, PY;Chen, KS;Zhang, PY
通讯作者地址：[Chen, KS; Zhang, PY]Shandong Univ, Natl Glycoengn Res Ctr, 72 Binhai Rd, Qingdao 266237, Peoples R China;[Chen, KS; Zhang, PY]Shandong Univ, Sch Life 更多
来源：BIOMEDICINE & PHARMACOTHERAPY
关键词：Polysaccharide; Colitis; Colitis-associated cancer; Mouse model;; Antitumor effect
摘要：An extracellular polysaccharide (EPS1-1) of Rhizopus nigricans was found to enhance immunity and reduce colon cancer cell proliferation. Here, the effect of EPS1-1 on a mouse model of colitis-associated cancer (CAC) induced by azoxymethane (AOM)/dextran sodium sulfate (DSS) was investigated. Pathological symptoms, including weight loss, piloerection, hematochezia and insensitivity caused by AOM/DSS, were relieved by EPS1-1. Anatomical results showed a 100% tumor incidence, a series of neoplasms, disordered cell structure and hyperplastic glands in the model group, while the abnormal behaviors were relieved and the tumors decreased in the EPS1-1 group. Compared with the model group, the EPS1-1 group showed decreased oncogenic protein (COX-2, beta-catenin, CyclinD1 and C-Myc) expression. TUNEL staining showed that EPS1-1 increased the apoptosis of colon cancer cells in mice. Furthermore, the expression of proliferative proteins (Ki-67 and PCNA) and an antiapoptotic gene transcript (Bcl-2) were significantly down regulated by EPS1-1, while apoptotic gene transcripts (p53 and Bax) were enhanced. In addition, EPS1-1 notably decreased the number of cells positive for CD68, F4/80 and NF-kappa B and reduced the concentrations of inflammatory factors (TNF-alpha and IL-6) in serum compared with those in the model group. Taken together, these results suggest that EPS1-1 may be a therapeutic option for the prevention and treatment of CAC.