标题：Spectroscopic Identification of Interactions of Formaldehyde with Bovine Serum Albumin
作者：Liu, Yihong; Liu, Rutao; Mou, Yue; Zhou, Guangjun
作者机构：[Liu, Yihong; Liu, Rutao; Mou, Yue] Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Peoples R China.; [Zhou, Guangjun] Shandong Univ, State Ke 更多
通讯作者地址：[Liu, RT]Shandong Univ, Sch Environm Sci & Engn, Jinan 250100, Peoples R China.
来源：JOURNAL OF BIOCHEMICAL AND MOLECULAR TOXICOLOGY
关键词：Bovine serum albumin; Formaldehyde; Interaction; Conformation;; Fluorescence
摘要：The mechanism of formaldehyde-protein interactions was investigated by determining the effects of formaldehyde on the common protein bovine serum albumin (BSA). The effects at the molecular level were determined by fluorescence, ultraviolet absorption, and circular dichroism (CD) spectrometry. Formaldehyde could decrease the amount of alpha-helix, leading to loosening of the protein skeleton. In the loose structure, internal amino acids are exposed and the characteristic fluorescence of BSA is obviously quenched. The spectroscopic results reveal that formaldehyde exposure induces changes in the microenvironment and conformation of serum albumin, which could lead to toxic effects on the organism. (C) 2010 Wiley Periodicals, Inc. J Biochem Mol Toxicol 25:95-100, 2011; View this article online at wileyonlinelibrary.com. DOI 10:1002/jbt.20364